Detection of highly pathogenic influenza and pandemic influenza virus in formalin fixed tissues by immunohistochemical methods
Identifieur interne : 002160 ( Main/Exploration ); précédent : 002159; suivant : 002161Detection of highly pathogenic influenza and pandemic influenza virus in formalin fixed tissues by immunohistochemical methods
Auteurs : John M. Nicholls [République populaire de Chine] ; Linda P. W. Wong [République populaire de Chine] ; Renee W. Y. Chan [République populaire de Chine] ; Leo L. M. Poon [République populaire de Chine] ; Leo K. Y. So [République populaire de Chine] ; Hui-Ling Yen [République populaire de Chine] ; Kevin Fung [République populaire de Chine] ; Sjouke Van Poucke [Belgique] ; J. S. Malik Peiris [République populaire de Chine]Source :
- Journal of virological methods [ 0166-0934 ] ; 2012.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
Tissues infected with highly pathogenic avian influenza viruses such as H5N1 and H7N7 are normally required to be fixed in formalin or paraformaldehyde before examination in order to inactivate the virus. In this study commercially available monoclonal antibodies to the influenza nucleoprotein (NP) were evaluated in order to determine which antibodies would identify positive cells in tissues fixed in formalin or paraformaldehyde. An assessment of which antigen retrieval process would unmask antigens blocked by formalin fixation was also made. Of six commercially available monoclonal antibodies tested, only one (HB65, European Veterinary Laboratories) was able to identify all formalin fixed avian, swine and human influenza virus infected tissues, and this was after pronase induced epitope retrieval. This monoclonal antibody is recommended for routine diagnostic use for the detection of influenza A infected tissues that have been fixed in formalin or paraformaldehyde.
Affiliations:
- Belgique, République populaire de Chine
- Province de Flandre-Orientale, Région flamande
- Gand
- Université de Gand
Links toward previous steps (curation, corpus...)
- to stream PascalFrancis, to step Corpus: 000796
- to stream PascalFrancis, to step Curation: 001640
- to stream PascalFrancis, to step Checkpoint: 000684
- to stream Main, to step Merge: 002220
- to stream Main, to step Curation: 002160
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Detection of highly pathogenic influenza and pandemic influenza virus in formalin fixed tissues by immunohistochemical methods</title><author><name sortKey="Nicholls, John M" sort="Nicholls, John M" uniqKey="Nicholls J" first="John M." last="Nicholls">John M. Nicholls</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Pathology, The University of Hong Kong, Pok Fu Lam</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hong Kong SAR</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Centre of Influenza Research, The University of Hong Kong</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hong Kong SAR</wicri:noRegion></affiliation></author><author><name sortKey="Wong, Linda P W" sort="Wong, Linda P W" uniqKey="Wong L" first="Linda P. W." last="Wong">Linda P. W. Wong</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Pathology, The University of Hong Kong, Pok Fu Lam</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hong Kong SAR</wicri:noRegion></affiliation></author><author><name sortKey="Chan, Renee W Y" sort="Chan, Renee W Y" uniqKey="Chan R" first="Renee W. Y." last="Chan">Renee W. Y. Chan</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Pathology, The University of Hong Kong, Pok Fu Lam</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hong Kong SAR</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Centre of Influenza Research, The University of Hong Kong</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hong Kong SAR</wicri:noRegion></affiliation></author><author><name sortKey="Poon, Leo L M" sort="Poon, Leo L M" uniqKey="Poon L" first="Leo L. M." last="Poon">Leo L. M. Poon</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Centre of Influenza Research, The University of Hong Kong</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hong Kong SAR</wicri:noRegion></affiliation></author><author><name sortKey="So, Leo K Y" sort="So, Leo K Y" uniqKey="So L" first="Leo K. Y." last="So">Leo K. Y. So</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Pathology, The University of Hong Kong, Pok Fu Lam</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hong Kong SAR</wicri:noRegion></affiliation></author><author><name sortKey="Yen, Hui Ling" sort="Yen, Hui Ling" uniqKey="Yen H" first="Hui-Ling" last="Yen">Hui-Ling Yen</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Centre of Influenza Research, The University of Hong Kong</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hong Kong SAR</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>The HKU-Pasteur Research Centre</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>6 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>The HKU-Pasteur Research Centre</wicri:noRegion></affiliation></author><author><name sortKey="Fung, Kevin" sort="Fung, Kevin" uniqKey="Fung K" first="Kevin" last="Fung">Kevin Fung</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Pathology, The University of Hong Kong, Pok Fu Lam</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hong Kong SAR</wicri:noRegion></affiliation></author><author><name sortKey="Van Poucke, Sjouke" sort="Van Poucke, Sjouke" uniqKey="Van Poucke S" first="Sjouke" last="Van Poucke">Sjouke Van Poucke</name><affiliation wicri:level="4"><inist:fA14 i1="04"><s1>Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University</s1><s2>Merelbeke</s2><s3>BEL</s3><sZ>8 aut.</sZ></inist:fA14><country>Belgique</country><placeName><settlement type="city">Gand</settlement><region>Région flamande</region><region type="district" nuts="2">Province de Flandre-Orientale</region></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Malik Peiris, J S" sort="Malik Peiris, J S" uniqKey="Malik Peiris J" first="J. S." last="Malik Peiris">J. S. Malik Peiris</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Centre of Influenza Research, The University of Hong Kong</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hong Kong SAR</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>The HKU-Pasteur Research Centre</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>6 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>The HKU-Pasteur Research Centre</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">12-0082227</idno><date when="2012">2012</date><idno type="stanalyst">PASCAL 12-0082227 INIST</idno><idno type="RBID">Pascal:12-0082227</idno><idno type="wicri:Area/PascalFrancis/Corpus">000796</idno><idno type="wicri:Area/PascalFrancis/Curation">001640</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000684</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000684</idno><idno type="wicri:doubleKey">0166-0934:2012:Nicholls J:detection:of:highly</idno><idno type="wicri:Area/Main/Merge">002220</idno><idno type="wicri:Area/Main/Curation">002160</idno><idno type="wicri:Area/Main/Exploration">002160</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Detection of highly pathogenic influenza and pandemic influenza virus in formalin fixed tissues by immunohistochemical methods</title><author><name sortKey="Nicholls, John M" sort="Nicholls, John M" uniqKey="Nicholls J" first="John M." last="Nicholls">John M. Nicholls</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Pathology, The University of Hong Kong, Pok Fu Lam</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hong Kong SAR</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Centre of Influenza Research, The University of Hong Kong</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hong Kong SAR</wicri:noRegion></affiliation></author><author><name sortKey="Wong, Linda P W" sort="Wong, Linda P W" uniqKey="Wong L" first="Linda P. W." last="Wong">Linda P. W. Wong</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Pathology, The University of Hong Kong, Pok Fu Lam</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hong Kong SAR</wicri:noRegion></affiliation></author><author><name sortKey="Chan, Renee W Y" sort="Chan, Renee W Y" uniqKey="Chan R" first="Renee W. Y." last="Chan">Renee W. Y. Chan</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Pathology, The University of Hong Kong, Pok Fu Lam</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hong Kong SAR</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Centre of Influenza Research, The University of Hong Kong</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hong Kong SAR</wicri:noRegion></affiliation></author><author><name sortKey="Poon, Leo L M" sort="Poon, Leo L M" uniqKey="Poon L" first="Leo L. M." last="Poon">Leo L. M. Poon</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Centre of Influenza Research, The University of Hong Kong</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hong Kong SAR</wicri:noRegion></affiliation></author><author><name sortKey="So, Leo K Y" sort="So, Leo K Y" uniqKey="So L" first="Leo K. Y." last="So">Leo K. Y. So</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Pathology, The University of Hong Kong, Pok Fu Lam</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hong Kong SAR</wicri:noRegion></affiliation></author><author><name sortKey="Yen, Hui Ling" sort="Yen, Hui Ling" uniqKey="Yen H" first="Hui-Ling" last="Yen">Hui-Ling Yen</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Centre of Influenza Research, The University of Hong Kong</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hong Kong SAR</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>The HKU-Pasteur Research Centre</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>6 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>The HKU-Pasteur Research Centre</wicri:noRegion></affiliation></author><author><name sortKey="Fung, Kevin" sort="Fung, Kevin" uniqKey="Fung K" first="Kevin" last="Fung">Kevin Fung</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Pathology, The University of Hong Kong, Pok Fu Lam</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hong Kong SAR</wicri:noRegion></affiliation></author><author><name sortKey="Van Poucke, Sjouke" sort="Van Poucke, Sjouke" uniqKey="Van Poucke S" first="Sjouke" last="Van Poucke">Sjouke Van Poucke</name><affiliation wicri:level="4"><inist:fA14 i1="04"><s1>Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University</s1><s2>Merelbeke</s2><s3>BEL</s3><sZ>8 aut.</sZ></inist:fA14><country>Belgique</country><placeName><settlement type="city">Gand</settlement><region>Région flamande</region><region type="district" nuts="2">Province de Flandre-Orientale</region></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Malik Peiris, J S" sort="Malik Peiris, J S" uniqKey="Malik Peiris J" first="J. S." last="Malik Peiris">J. S. Malik Peiris</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Centre of Influenza Research, The University of Hong Kong</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hong Kong SAR</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>The HKU-Pasteur Research Centre</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>6 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>The HKU-Pasteur Research Centre</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Journal of virological methods</title><title level="j" type="abbreviated">J. virol. methods</title><idno type="ISSN">0166-0934</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of virological methods</title><title level="j" type="abbreviated">J. virol. methods</title><idno type="ISSN">0166-0934</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Detection</term><term>Immunohistochemistry</term><term>Influenza</term><term>Influenzavirus</term><term>Method</term><term>Nucleoprotein</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Influenzavirus</term><term>Détection</term><term>Immunohistochimie</term><term>Méthode</term><term>Nucléoprotéine</term><term>Grippe</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Tissues infected with highly pathogenic avian influenza viruses such as H5N1 and H7N7 are normally required to be fixed in formalin or paraformaldehyde before examination in order to inactivate the virus. In this study commercially available monoclonal antibodies to the influenza nucleoprotein (NP) were evaluated in order to determine which antibodies would identify positive cells in tissues fixed in formalin or paraformaldehyde. An assessment of which antigen retrieval process would unmask antigens blocked by formalin fixation was also made. Of six commercially available monoclonal antibodies tested, only one (HB65, European Veterinary Laboratories) was able to identify all formalin fixed avian, swine and human influenza virus infected tissues, and this was after pronase induced epitope retrieval. This monoclonal antibody is recommended for routine diagnostic use for the detection of influenza A infected tissues that have been fixed in formalin or paraformaldehyde.</div></front></TEI><affiliations><list><country><li>Belgique</li><li>République populaire de Chine</li></country><region><li>Province de Flandre-Orientale</li><li>Région flamande</li></region><settlement><li>Gand</li></settlement><orgName><li>Université de Gand</li></orgName></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Nicholls, John M" sort="Nicholls, John M" uniqKey="Nicholls J" first="John M." last="Nicholls">John M. Nicholls</name></noRegion><name sortKey="Chan, Renee W Y" sort="Chan, Renee W Y" uniqKey="Chan R" first="Renee W. Y." last="Chan">Renee W. Y. Chan</name><name sortKey="Chan, Renee W Y" sort="Chan, Renee W Y" uniqKey="Chan R" first="Renee W. Y." last="Chan">Renee W. Y. Chan</name><name sortKey="Fung, Kevin" sort="Fung, Kevin" uniqKey="Fung K" first="Kevin" last="Fung">Kevin Fung</name><name sortKey="Malik Peiris, J S" sort="Malik Peiris, J S" uniqKey="Malik Peiris J" first="J. S." last="Malik Peiris">J. S. Malik Peiris</name><name sortKey="Malik Peiris, J S" sort="Malik Peiris, J S" uniqKey="Malik Peiris J" first="J. S." last="Malik Peiris">J. S. Malik Peiris</name><name sortKey="Nicholls, John M" sort="Nicholls, John M" uniqKey="Nicholls J" first="John M." last="Nicholls">John M. Nicholls</name><name sortKey="Poon, Leo L M" sort="Poon, Leo L M" uniqKey="Poon L" first="Leo L. M." last="Poon">Leo L. M. Poon</name><name sortKey="So, Leo K Y" sort="So, Leo K Y" uniqKey="So L" first="Leo K. Y." last="So">Leo K. Y. So</name><name sortKey="Wong, Linda P W" sort="Wong, Linda P W" uniqKey="Wong L" first="Linda P. W." last="Wong">Linda P. W. Wong</name><name sortKey="Yen, Hui Ling" sort="Yen, Hui Ling" uniqKey="Yen H" first="Hui-Ling" last="Yen">Hui-Ling Yen</name><name sortKey="Yen, Hui Ling" sort="Yen, Hui Ling" uniqKey="Yen H" first="Hui-Ling" last="Yen">Hui-Ling Yen</name></country><country name="Belgique"><region name="Région flamande"><name sortKey="Van Poucke, Sjouke" sort="Van Poucke, Sjouke" uniqKey="Van Poucke S" first="Sjouke" last="Van Poucke">Sjouke Van Poucke</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/PandemieGrippaleV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002160 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002160 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= PandemieGrippaleV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= Pascal:12-0082227
|texte= Detection of highly pathogenic influenza and pandemic influenza virus in formalin fixed tissues by immunohistochemical methods
}}
| This area was generated with Dilib version V0.6.34. |  |